TECHNOLOGY PLATFORMS

Advanced Protein Expression Systems

Four complementary platforms to meet any protein challenge. From simple antigens to complex membrane proteins.

OUR PLATFORMS

Four Expression Systems

🧬

E. coli Expression

Fast, high-yield, cost-effective. Ideal for non-glycosylated proteins, antigens, and small peptides.

mg to multi-gram scale
>95% success rate
2-3 weeks standard delivery
Soluble & inclusion body refolding
His/GST/MBP tag options
Endotoxin removal available
Typical applications: Antigens, enzymes, cytokines, vaccine candidates.
🧪

Yeast Expression (Pichia pastoris)

Secreted expression with eukaryotic post-translational modifications. High-density fermentation.

High yield (g/L)
Glycosylation capable
3-4 weeks delivery
Secreted or intracellular
cGMP available
Cost-effective for scale-up
Typical applications: Enzymes, allergens, single-chain antibodies, hormones.
🦋

Insect Cell Expression (Baculovirus)

High-level expression of complex, properly folded eukaryotic proteins with native-like modifications.

Up to 500 mg/L yield
Complex PTMs
4-6 weeks delivery
Kinases, membrane proteins
Multi-protein complexes
Virus-like particles (VLPs)
Typical applications: Kinases, GPCRs, ion channels, viral antigens.
🐁

Mammalian Expression (HEK293/CHO)

Native folding and authentic post-translational modifications. Transient and stable cell line generation.

Native glycosylation
Proper folding & activity
4-8 weeks delivery
Antibodies, cytokines
Stable pools/clones
Low endotoxin
Typical applications: Monoclonal antibodies, Fc-fusion proteins, therapeutic candidates.
AT A GLANCE

Expression System Comparison

FeatureE. coliYeastInsect CellMammalian
Yield (typical)High (g/L)High (g/L)Moderate (mg/L)Low-Moderate
GlycosylationNoYes (high mannose)Yes (simple)Yes (complex)
Turnaround2-3 weeks3-4 weeks4-6 weeks4-8 weeks
Cost$$$$$$$$$$
Best forAntigens, enzymesSecreted proteinsKinases, VLPsAntibodies

Not sure which system to choose? Contact our experts for a free consultation.

TECHNICAL FAQ

Common Questions

Which system is best for my protein?

We provide a free feasibility assessment based on your protein's properties. Contact us with your sequence or target details.

Can you handle difficult-to-express proteins?

Yes. We have extensive experience with membrane proteins, toxic proteins, and aggregation-prone targets.

Do you offer endotoxin removal?

Yes, endotoxin removal is available for all platforms. We can achieve <0.1 EU/µg for sensitive applications.

Can you scale up my protein production?

Absolutely. We offer seamless scale-up from mg to gram quantities, including fermentation optimization.